Fragmentation is caused by creating and deleting files and folders, 
installing new software, and downloading files from the Internet. 
Computers do not necessarily save an entire file or folder in a single 
space on a disk; they're saved in the first available space. After a 
large portion of a disk has been used, most of the subsequent files and 
folders are saved in pieces across the volume.
When you delete files 
or folders, the empty spaces left behind are filled in randomly as you 
store new ones. This is how fragmentation occurs. The more fragmented 
the volume is, the slower the computer's file input and output 
performance will be.
Defragmentation is the process of rewriting 
non-contiguous parts of a file to contiguous sectors on a disk for the 
purpose of increasing data access and retrieval speeds. Because FAT and 
NTFS disks can deteriorate and become badly fragmented over time, 
defragmentation is vital for optimal system performance.
Just extract onto USB and run.
Tested and work on Windows XP SP3.
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